Accurate and selective quantification of anthrax protective antigen in plasma by immunocapture and isotope dilution mass spectrometry
نویسندگان
چکیده
منابع مشابه
Quantification of cardiovascular biomarkers in patient plasma by targeted mass spectrometry and stable isotope dilution.
Verification of candidate biomarkers requires specific assays to selectively detect and quantify target proteins in accessible biofluids. The primary objective of verification is to screen potential biomarkers to ensure that only the highest quality candidates from the discovery phase are taken forward into preclinical validation. Because antibody reagents for a clinical grade immunoassay often...
متن کاملMethylmalonic acid measured in plasma and urine by stable-isotope dilution and electrospray tandem mass spectrometry.
BACKGROUND Liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization is robust and allows accurate measurement of both low- and high-molecular weight components of complex mixtures. We developed a LC-MS/MS method for the analysis of methylmalonic acid (MMA), a biochemical marker for inherited disorders of propionate metabolism and acquired vitamin B(12) deficiency. ...
متن کاملQuantification of growth hormone in serum by isotope dilution mass spectrometry.
Interassay variation of antibody-based routine tests hampers comparability of measurement results for growth hormone (GH) between different laboratories and decision making in clinical practice. Here it is demonstrated that quantification of GH by isotope dilution mass spectrometry (IDMS) constitutes a way to obtain precise and reliable results that can be referred to in evaluation of performan...
متن کاملQuantification of saxitoxin and neosaxitoxin in human urine utilizing isotope dilution tandem mass spectrometry.
Saxitoxin and neosaxitoxin are potent neurotoxins that can cause paralytic shellfish poisoning when consumed. A new assay is presented here to quantify saxitoxin (STX) and neosaxitoxin (NEO) in human urine samples. Sample preparation of 500-microL samples included the use of weak-cation-exchange solid-phase extraction in a multiplexed 96-well format. Extracts were preconcentrated and analyzed v...
متن کاملIsotope-Dilution Mass Spectrometry for Quantification of Urinary Active Androgens Separated by Gas Chromatography
Cross reacting antibodies can cause an overestimation of the results of immunoassays. Therefore, alternative methods are needed for the accurate quantification of steroids. Gas chromatography combined with isotope-dilution mass spectrometry (GC-IDMS) is developed to quantify urinary active androgens, testosterone, epitestosterone and dihydrotestosterone, which are clinically relevant androgens ...
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ژورنال
عنوان ژورنال: The Analyst
سال: 2019
ISSN: 0003-2654,1364-5528
DOI: 10.1039/c8an02479k